Contagious agalactia (CA) is one of the main contagious diseases that affect small ruminants. The main causal agent is Mycoplasma agalactiae, a contagious bacterium that leads to chronic infections. Prophylaxis is considered to be the most viable, economical and sustainable method to combat this pathogen. There are currently several inactivated vaccines against M. agalactiae in Spain. The objective of this study was to compare the short-term and long-term serological response to different commercial vaccines against M.agalactiae available in Spain.