Diagnostic tool for the detection and quantification of pathogenic bacteria in bovine milk samples from dairy farms in Bangladesh

Publications

Cattle

Authors

Pochodyla, M.; Solaiman, M.

STARTCHECK® is a good diagnostic and surveillance tool to identify pathogenic bacteria present at a herd level using BTM and PMM samples. The high specificity and sensitivity circumvents limitations experienced with bacteriology (transport or poor growth) and the human error with individual cow SCC cut off level of 200 000 cells/ ml.

STARTCHECK® is a reliable diagnostic tool that uses a new methodology to collect milk samples and detect the major mastitis causative agents. STARTCHECK® can be used to complement Somatic Cell Count (SCC) and bacterial culture to monitor mastitis at a herd level1. STARTCHECK® has been used worldwide since 2009 and milk samples from 44 different countries have been analysed. The aims of the present study were: 1) to show the results obtained from milk samples from Bangladesh dairy farms, using an FTA card and PCR diagnostic tool (STARTCHECK®) provided by HIPRA and 2) to show that STARTCHECK® can be used to complement Somatic Cell Count (SCC) and Bacterial culture to monitor mastitis at a herd level by detecting the major mastitis causative agents [Staphylococcus aureus, Escherichia coli, Staph. Sp. (Coagulase Negative Staphylococci) and coliform bacteria].