STARTCHECK® is a reliable diagnostic tool that uses a new methodology to collect milk samples and detect the major mastitis causative agents. STARTCHECK® can be used to complement Somatic Cell Count (SCC) and bacterial culture to monitor mastitis at a herd level1. STARTCHECK® has been used worldwide since 2009 and milk samples from 44 different countries have been analysed. The aims of the present study were: 1) to show the results obtained from milk samples from Bangladesh dairy farms, using an FTA card and PCR diagnostic tool (STARTCHECK®) provided by HIPRA and 2) to show that STARTCHECK® can be used to complement Somatic Cell Count (SCC) and Bacterial culture to monitor mastitis at a herd level by detecting the major mastitis causative agents [Staphylococcus aureus, Escherichia coli, Staph. Sp. (Coagulase Negative Staphylococci) and coliform bacteria].