BOVIRESP® is a diagnostic tool to identify rapidly and accurately the main viruses involved in a respiratory outbreak. Nasal exudates from calves from Spanish feedlots have been analyzed by real time PCR and the results obtained during a 5 years period showed a 51%, 47% and 13% of prevalence of BVDV, BRSV and IBRV, respectively, among the positive RT-PCR samples. BOVIRESP® has been established as an important tool to support cattle veterinarians.