The aims of the present study were to determine the apparent prevalence of Staphylococcus aureus, Escherichia coli and other coliforms (COL) from bulk tank milk (BTM) samples of dairy farms from Azores, to evaluate the management milking practices associated with these pathogens prevalence, and consequently, the ultimate goal was to emphasize the indication, in Azorean dairy farms, for the use of the first vaccine registered by EMA (European Medicine Agency) for the prevention of mastitis caused by these pathogens (STARTVAC®, HIPRA, Spain).