Indirect immunofluorescence (IFAT) is the reference technique for the diagnosis of Canine leishmaniasis, but it is also complicated as it requires expertise for its interpretation. ELISA is an alternative technique which offers a suitable diagnostic performance and easy use and interpretation. CIVTEST® CANIS LEISHMANIA is an indirect ELISA, which shows an excellent level of correlation to IFAT. The objective of this work is to adapt CIVTEST® CANIS to a high throughput automated ELISA platform.

Indirect immunofluorescence (IFAT) is the reference technique for the diagnosis of Canine leishmaniasis, but it is also complicated as it requires expertise for its interpretation. ELISA is an alternative technique which offers a suitable diagnostic performance and easy use and interpretation. CIVTEST® CANIS LEISHMANIA is an indirect ELISA, which shows an excellent level of correlation to IFAT. The objective of this work is to adapt CIVTEST® CANIS to a high throughput automated ELISA platform.