The presumptive clinicpathological diagnosis of atrophic rhinitis (AR) is confirmed by demonstration of its causative agents, Bordetella bronchiseptica (Bb) and toxigenic strains of Pasteurella multocida (PMT), by means of culture or PCR from nasal or tonsil swabs or biopsies. Given that pig oral fluid (OF) is being increasingly used as a diagnostic specimen in pigs, the objective of this research was to evaluate its potential use as a sample for the diagnosis and surveillance of AR, coupled with PCR.